[That is a very interesting question. He actually has to mull that over for a few seconds.]

Every living being in my world has chakra - whether they've accessed it or even know of its existence is another story, but we all have it. I wouldn't know if there are people here who don't have chakra, but I would hazard a guess that it would matter very little either way. Itachi might not get anything off of them, but if you're not fast enough to keep up with him it's not going to matter.